Cell microarray in detection of serine protease expression in Cryptococcus neo formans
- Author:
Chi-Yu XU
1
Author Information
1. Department of Dermatology and Venereology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(2):129-131
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine the serine protease expression in Cryptococcus neoformans (C. neoformans) by cell microarray technique,so as to investigate the role of serine protease in the pathogenesis of C. neoformans. Methods: The cell microarray was constructed with tissue microarray. Thirty-six strains of C. neoformans of different sources and homologous serotypes were examined for their serine protease expression by cell microarray technique and immunohistochemistry. Results: Strong expressi on of serine protease was found in 25(67.0%) strains. The rates of strong serine protease expression in serotype A, B and D/AD strains were 46.2%(6/13), 92.3%(12/13) and 66.7%(4/6), and in environment-isolated, clinically isolated and uncapsuled strains were 55.6%(5/9),82.6%(19/23) and 25%(1/4), respectively. Serine protease expressions in serotype B and clinically-isolated strains were significantly higher than that in other group (P<0.05). Conclusion: Mic roarray of strain cells is a new method for identifying pathogenic fungus. Higher expression of serine protease in clinically-isolated strains is associated with strong virulence of clinical isolates strains, suggesting that serine protease plays a major role in the pathogenesis of C. neoformans.
