Study on enrichment and purification of astragalosides IV from Radix astragali with macroporous resins
- Author:
Yue GAO
1
Author Information
1. School of Pharmacy
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(3):334-336
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the enrichment and purification method of astragaloside IV from Radix astragali with macroporous resin, so as to lay a foundation for the large scale production of astragaloside IV. Methods: A HPLC-MSD method was established to determine the content of astragaloside IV in Radix astragali. The adsorption ability and capability of different macroporous resins were investigated. The optimal alcohol concentration and capability of alcohol for elution were also confirmed through the determination of astragaloside IV in the eluted solution. Results: HPLC-MSD achieved satisfactory results in determination of astragaloside IV. D101 was proven to be the most potent macroporous resin; its adsorption capability of astragaloside IV was 0.42 mg/g when the elution solution was 5 folds of 70% alcohol. Conclusion: D101 macroporous resin can be used to effectively enrich and purify astragaloside IV, laying a foundation for analyzing the constituents of total astragalosides.
