HPLC-ELSD in determination of AR-I content in total furostanol saponins of Asparagus cochinchinensis
- Author:
Cong-Li XU
1
Author Information
1. Department of Natural Medicinal Chemistry
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(4):427-428
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine AR-I content in total furostanol saponins of Asparagus cochinchinensis. Methods: High performance liquid chromatogra phy (HPLC) was used and the condition was: Diamonsil C18 column (5 mm×250 mm, 5 μm); mobile phase: CH3CN-H2O (36 : 64); flow rate: 0.8 ml/min; injector volume:20 μl;colum temperature: room temperature. Evaporative light scattering detector(ELSD) condition was: drift tube temperature: 40°C; and nebulizer gas pressure (N2): 2.0×105 Pa. Results: The linearity was good when AR-I was within the range of 0.094-0.940 0 g · L-1 (r=0.999 2). The test had higher sensitivity and good stability. The average recovery was 101.3% (n=6)and RSD was 2.36%. Conclusion: HPLC-ELSD is practical and reliable for the determination of the furostanol saponins of Asparagus cochinchinensis.
