Hepatic gene expression profile early after 60Co-γ irradiation in mice
- Author:
Li-Hua SONG
1
Author Information
1. Department of Food Science and Engineering
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(4):386-390
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the hepatic gene expression profile early after 60Co-γ irradiaiton in mice with cDNA microarray, in an attempt to understand the molecular mechanism of irradiation damage to liver in mice. Methods: Total RNA was extracted from mice liver 2 days after irradiation with 60Co-γ ray and from normal mice liver. Hepatic gene expression patterns of irradiated and normal mice were compared with cDNA microarray(4 096 genes). RT-PCR was performed to validate the cDNA microarray results. Results: Compared with normal group, 124 genes showed a differential expression in irradiation group, with 46 genes upregulated(maily cell cycle and transcription regulation related proteins) and 78 down-regulated(mainly cytoskeletal genes). Of the 124 genes, 57 had identified functions and could be divided into 6 groups: DNA repair and stress response, cytoskeleton, hydronium channel/transport protein, signaling transduction, intermediary metabolism, and immune related proteins. RT-PCR analysis indicated that the expression of heat shock 70kD protein 5 (Hspa5), RAS p21 protein activator 3 (Rasa3), and NAD(P) H dehydrogenase, quinone 1 (Nqo1) were consistent with cDNA microarray data. Conclusion: Irradiation-induced hepatic injury is of multi-target and multi-pathway.
