Two-dimensional electrophoresis in proteomic comparison and indentification of brain stem proteins in seasickness adaptive and non-adaptive rats
- Author:
Bo SU
1
Author Information
1. Department of Military Hygiene
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(4):382-385
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a two-dimensional eletrophoresis system for total brain stem proteins of seasickness adaptive and non-adaptive rats and to identify the differentially expressed proteins for investigation of the possible mechanism of seasickness adaptation. Methods: According to the kaolin intakes after seasickness stimulation, 30 rats were divided into non-seasickness group (n=10), seasickness adaptive group (n=12) and seasickness non-adaptive group (n=8). Another 10 normal rats not receiving stimulation were taken as blank control. The brain stem proteins of seasickness adaptive and seasickness non-adaptive rats (n=6) were isolated by two-dimensional electrophoresis; the differentially expressed proteins were identified by peptide mass fingerprint (PMF). Results: During a 21-day stimulation, the amount of kaolin consumption in seasickness adaptive rats experienced an increasing phase and a subsequent decreasing phase; seasickness non-adaptive rats consumed significantly more kaolin during the whole 21-day stimulation than the blank control rats did (P<0.01 or P<0.05). Nine seasickness adaptive proteins were identified by PMF: peroxiredoxin I, peroxiredoxin II, light molecular-weight neurofilament, ubiquitin carboxyl-terminal hydrolase PGP9.5, and glutamine synthetase were highly expressed; carbonic anhydrase II, triosephosphate isomerase I, phosphoglycerate mutase isozyme B and mitochondrial voltage dependent anion channel were lowly expressed. Conclusion: Adaptation to seasickness can induce changes in the protein expression pattern of brain stem in the seasickness adaptive rats, which may be associated with energy metabolism, neurotransmitter adjustment and oxidative stress.
