Interaction between downstream of kinases 1 and TrkA; a yeast two-hybrid approach
- Author:
Xuan LIU
1
Author Information
1. Department of Neurobiology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(5):497-499
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the interaction between TrkA and downstream of kinases 1(dok 1) and search for the possible intracellular substrates or regulatory proteins of TrkA, so as to better understand the mechanism of downstream signal transduction of TrkA. Methods: The TrkAIC (intracellular domain of TrkA) was fused with LexA and the product was used as a DNA-binding domain protein; dok1, dok1PTB and dok1ΔPTB(dok1 without PTB) were separately fused with B42AD and their products were used as activation domain protein. Cotransformants were subjected to β-galactosidase activity analysis and leucine medium growth analysis for understanding of their interaction. Results: Dok1 and dok1PTB interacted with TrkA in yeast, while dok1ΔPTB did not. Conclusion: It is confirmed that TrkA can interact with dok1 in yeast, and the interaction may be mediated by PTB domain of dok1.
