Comparison of morphology and growth kinetics of adult normal and herniated nucleus pulposus cells cultured in vitro
- Author:
Rong-Feng ZHANG
1
Author Information
1. Department of Orthopaedics
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(5):479-483
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the morphology and growth kinetics of the cells derived from the adult normal and herniated nucleus pulposus tissues. Methods: The cells derived from the adult normal and herniated nucleus pulposus were cultured in vitro. Passage 1,3,5 cells were chosen for light microscope and electron microscope observation. The cell growth rate, MTT value, maximal growth density, cellular mitotic index, cloning efficiency, and other parameters were observed in normal and herniated nucleus pulposus and the results were compared. Results: Compared with adult normal nucleus pulposus cells, the cells isolated from herniated nucleus pulposus were irregularly arranged and lost polarization, with rough endoplasmic reticulum slightly dilated. Under the same cellular density and culture conditions, the growth rate of passage 1 cells from adult normal and herinated nucleus pulposus had no significant difference, but the differences of passage 3 cells(P<0.01) and passage 5 cells (P<0.05) were statistically significant. The maximal growth density, MTT value, cellular mitotic index, and cloning efficiency of passage 1 cells of adult normal and herinated nucleus pulposus were respectively 7.28±0.23 and 7.41±0.17, 0.186±0.015 and 0.173±0.008, (25.36±2.14)‰ and (23.13±2.55)‰, and (52.4±2.8)% and (47.7±2.6)% (all P>0.05); those of passage 3 cells were respectively 6.96±0.19 and 5.48±0.22, 0.154±0.018 and 0.110±0.009, (22.52±2.16)‰ and (16.68±2.35)‰, and (48.7±3.3)% and (40.7±2.5)%; and of passage 5 cells were respectively 2.42±0.13 and 0.98±0.14, 0.085±0.006 and 0.050±0.007, (15.61±1.96)‰ and (9.34±2.12)‰, and (40.2±2.3)% and (28.34±2.9)%. There were significant differences between the parameters of passage 3 and 5 cells(P<0.05). Conclusion: The adult normal and herinated nucleus pulposus cells are different in cellular morphology when cultured in vitro. The continuous proliferation ability of the herniated nucleus pulposus cells is obviously decreased than that of the adult normal control group, and aging is more easily seen in the herniated ones.
