An acute lung injury model caused by seawater aspiration in rats
- Author:
Yu SUN
1
Author Information
1. Department of Pathophysiology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(6):676-678
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an acute lung injury model by seawater aspiration in rats. Methods: Twenty-four rats were randomly allocated i nto 2 groups: control group (n=9) and model group (n=15). The acute lung injury model was established in rats by aspirating 2 ml seawater/kg body weight after 9-10 seconds of breath occlusion. The aspiration was repeated by the same means 2 min later. Rats in control group aspirated no seawater. Arterial blood gases and respiratory rates were observed before aspiration and 10 min, 30 min, 1 h, 2 h and 4 h after aspiration. The survival times of animal were also recorded. The rats were sacrificed 4 h after aspiration; the lung index, total protein (TP) contents, white blood cell (WBC) count in bronchoalveolar lavage fluid (BALF), and the pulmonary histopathology were observed. Results: Compared with control group, model group had a significantly increased respiratory rate and a decreased PaO2 (P<0.05), which subsequently rose again and remained at a lower level. The model group also had a markedly increased lung index, TP contents and WBC counts (P<0.05). Obvious edema, alveolar atrophy and inflammatory infiltration were found in model rats. Conclusion: An acute lung injury model by sea water aspiration has been successfuly established.
