Heparin-enzyme linked immunosorbent assay in determination of serum midkine concentration and its application in diagnosis of hepatocellular carcinoma
- Author:
Jin LI
1
Author Information
1. Department of Molecular Oncology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(6):631-633
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop a method for determination of serum midkine (MK) concentration and to evaluate its value in the diagnosis of hepatocellular carcinoma (HCC). Methods: Since heparin can bind to MK, a heparin-enzyme linked immunosorbent assay (ELISA) for determination of MK was developed using heparin-antibody sandwich technique. Serum MK levels were measured in 20 healthy adults, 20 patients with benign liver tumors, 20 with liver cirrhosis, and 104 with HCC. Results: The method had a minimum detectab le concentration of 15 ng/L and a detectable range of (15-1 000) ng/L, and a linearity was found when the concentration was <500 ng/L(r=0.97). Serum MK levels of the healthy subjects, the patients with benign liver tumor, liver cirrhosis and HCC were (56±11) ng/L, (74±19) ng/L, (89±43) ng/L and (199±87) ng/L, respectively; with significant difference found between HCC group and the former 3 groups (P<0.05). The positive rate of MK in HCC group was 83.7%, significantly higher than those of 3 other groups (P<0.001). Conclusion: The heparin-ELISA method for MK determination has diagnostic value for HCC.
