Study on interaction between suc1-binding neurotrophic target 1 and RET by yeast two-hybrid method
- Author:
Li JIAO
1
Author Information
1. Department of Neurobiology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(6):617-619
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the interaction between RET (GDNF receptor) with sucl-bingding neurotrophic target (SNT1) and search for the possible downstream substrates or regulatory proteins of RET, so as to reveal the mechanism of downstream signal transduction of RET. Methods: The RETIC (intracellular domain of RET) was fused to LexA and the product was used as a DNA-binding domain protein. SNT1, SNT1PTB or SNT1ΔPTB (SNT1 without PTB) was separately fused to B42AD and their products were used as an activation domain protein. The cotransformants were tested by β-galactosidase activity analysis and leucine medium growth analysis was used to study the interaction between SNT1 and RET. Results: Interaction between RET and SNT1 or SNT1PTB was found in yeast, but that between SNT1ΔPTB and RET was not observed. Conclusion: It i s confirmed that RET can interact with SNT1 in yeast, and the interaction may be mediated by PTB domain of SNT1.
