Establishment and optimization of two-dimensional gel electrophoresis for separation of human kidney phosphoproteome
- Author:
Ya-Wei LIU
1
Author Information
1. Department of Nephrology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(6):603-606
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To separate human kidney phosphoproteome by two-dimensional gel electrophoresis. Methods: The phosphorylated proteins from human kidney tissues were enriched with phosphate metal affinity chromatography (PMAC) resin. After being concentrated and desalted, the samples were separated by isoelectric focusing on first dimension and SDS electrophoresis on second dimension. Results: The phosphorylated proteins were successfull y extracted from human kidney tissues and were separated by two-dimensional gel electrophoresis. Conclusion: Phosphoprotein enrichment technique combin ed with two-dimensional gel electrophoresis is an effective approach to study phosphoproteome, laying a foundation for further investigation of human kidney phosphoproteins.
