Protective effect of peroxisome proliferator-activated receptor γ on TNFα-induced injury of inner medullary collecting duct cells
- Author:
Qin LIN
1
Author Information
1. Department of Nephrology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(6):599-602
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the protective effect of peroxisome proliferator-activated receptor γ (PPARγ) on TNFα-induced injury of the inner medullary collecting duct (IMCD) cells. Methods: Cultured IMC D cells were randomly divided into blank control, TNFα stimulation, PPARγ transfection (pPPARγ-control) and PPARγ transfection + TNFα stimulation groups. Stimulation was induced with 50 ng/ml TNFα for 24 h and mouse wild-type PPARγ plasmid was used to transfect IMCD cells. Cell supernatant MCP-1 or TGFβ1 was detected by ELISA method. Results: IMCD cells transfected with wild-type PPARγ pla smid had high expression of PPARγ mRNA and protein. The contents of MCP-1 and TGFβ1 in the supernatant were similar in blank control group and pPPARγ-control group. Compared with blank control group, TNFα stimulation group had decreased contents of MCP-1 and TGFβ1 in the supernatant (P<0.005), but the contents of TNFα stimulation group were significantly higher than those of PPARγ transfection + TNFα stimulation group (P<0.05 and P<0.005, respectively). Conclusion: IMCD cells over-expressing PPARγ have anti-inflammatory and anti-fibrosis effects.
