Pretreatment of sarsasapogenin in Rhizoma Anemarrhenae: A research of methodology
- Author:
Dong-Liang ZHU
1
Author Information
1. Department of Drug Analysis
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(7):790-791
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To improve the pretreatment method described in ChP (2005 Edition, Vol I) for the determination of sarsasapogenin in Rhizoma Anemarrhenae. Methods: According to orthogonal design L9 (33), the extraction time, alcohol concentration, extraction frequency, etc. were modified to achieve the best extraction outcome with ultrasonic wave extraction. Results: The optimal extraction condition included 70% alcohol and twice ultrasonic wave extraction, 20 min each time. Finally hydrolization was applied to obtain sarsasapogenin for determination. Conclusion: Compared with the pretreatment method in ChP, this improved extraction method shows a higher sarsasapogenin content in determination and is more accurate in reflecting the true content of sarsasapogenin in Rhizoma Anemarrhene.
