Preparation of total isomalabaricanes and determination of Stelletin B and Stellettin D in Stelletta tenuis Lindgren by high pressure liquid chromatography
- Author:
Wei XIONG
1
Author Information
1. Marine Biotechnology Laboratory
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(7):787-789
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for determining the contents of Stellettin B and Stellettin D in total isomalabaricanes in different batches of Stelletta tenuis Lindgren. Methods: A high pressure liquid chromatography (HPLC) method was applied. The column YMC Pack-SIL (250 mm×4.6 mm, 5 μm) was used at room temperature, and the mobile phase was n-hexane: ethyl acetate (75:25) with a flow rate at 1.0 ml · min-1 for 30 min. UV detector wavelength was at 360 nm. Results: The regression equation were:Y=52 181 633 X+957 495 (r=0.996 9) for Stellettin B and Y=62 636 828 X-451 022 (r=0.998 0) for Stellettin D, with the linear range being 2.4-30 μg and 2.0-32 μg, correspondingly. The RSD values for accuracy test, stability test and repeatability test were 3.07%-4.76%. The average contents of Stellettin B and Stellettin D in total isomalabaricanes in different batches of Stelletta tenuis Lindgren were 59.93% and 30.98%, respectively. Conclusion: This HPLC method provides a fast, accurate and effective way to determine the active contents in total isomalabaricanes from Stelletta tenuis Lindgren.
