rhCD40L induces mononuclear macrophages secreting matrix metalloproteinases through cyclooxygenase-2 pathway
- Author:
Min FAN
1
Author Information
1. Department of Cardiovasology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(8):845-847
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the influence of rhCD40L on mononuclear macrophage, U937 cells, secreting matrix metalloproteinases (MMPs) and its possible mechanism. Methods: U937 cells were treated with different concentrations of rhCD40L and NS-398 (specific antagonist of COX-2) for 24 h and the supernatants were harvested. Cells treated with 0.4 μg/ml rhCD40L were further treated with 10-4 mol/L NS-398 or aspirin separately for 24 h and the supernatants were harvested. Zymography was used to determine the activities of MMPs in the above supernatants. Results: rhCD40L increase the activity of MMP-2 and MMP-9 in a dose-dependent manner (P<0.01); the increased peaked when rhCD40L was at 0.4 μg/ml. NS-398 inhibited the activity of MMP-2 and MMP-9 in a dose-dependent manner (P<0.05). NS-398 and aspirin both significantly inhibited the activity of MMP-2 and MMP-9 induced by rhCD40L (P<0.05). Conclusion: rhCD40L can induce U937 cells to secrete MMPs in a dose dependent manner, which might be related to COX, more possibly through COX-2 than COX-1 pathway.
