Construction of pancreatic duodenal homeobox-1 recombinant retroviral vector and its stable expression in liver progenitor cells
- Author:
Cai-Xia JIN
1
Author Information
1. Department of Cell Biology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(8):834-836
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To construct a recombinant retroviral vector expressing pancreatic duodenal homeobox-1 (PDX-1) gene and express the PDX-1 expression cassette in liver progenitor cells (LEPCs), so as to study the conversion of hepatic cells to pancreatic-like cells. Methods: The full length of PDX-1 gene was amplified by PCR and subcloned into pMSCVpuro vector. The pMSCV PDX-1 puro was then introduced into Phoenix package cells and the cell culture supernatant was used for Ping-Pong infection of another packaging cell line PT67 to obtain stable virus-producing cell line. Results: The pMSCV PDX-1 puro vector was successfully constructed and transfected into PT67 cells. The viral supernatants of PT67 cells efficiently infected LEPCs in vitro and the infected LEPCs stably expressed PDX-1 gene. Conclusion: The pMSCV PDX-1 puro vector and PDX-1 expressing LEPCs have been successfully constructed, laying a basis for studying the transdifferentiation of liver progenitor cells to pancreas lineage cells.
