Changes of serum IL-6 levels and pulmonary IL-6 mRNA expression after high-volume hemofiltration in pigs with multiple organ dysfunction syndrome
- Author:
Bing WANG
1
Author Information
1. Department of General Surgery
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(9):961-964
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of high-volume hemofiltration (HVHF) on serum levels of IL-6 and pulmonary expression of IL-6 mRNA in pigs with multiple organ dysfunction syndrome (MODS). Methods: Twenty-five pigs were randomly divided into model group, control group and HVHF group. Animals in control group were injected with normal saline, and those in the other 2 groups were treated with "a second hit" to establish MODS model, then animals in HVHF group was treated with HVHF for 3 days. Serum IL-6 levels were determined by ELISA and Real-time quantitative PCR was used to determine the expression level of IL-6mRNA. Lymphocyte apoptosis was detected by Annexin-V/PI flow cytometry. Results: After HVHF, the serum indices associated with organ-function and the morbidity and mortality were much lower than those in MODS model group (P<0.05). The expression level of IL-6 mRNA in lung was reduced significantly in HVHF group compared with that in model group (P<0.05); moreover, the lymphocyte apoptosis rate was also significantly decreased compared with model group (P<0.01). Conclusion: Early HVHF can decrease the high serum levels of IL-6 and the over-expression of IL-6 mRNA in lungs caused by trauma; it can also decrease the lymphocyte apoptosis rate, so as to prevent the incidence of lung failure.
