Protective effect of activated AMP-activated protein kinase on palmatic acid-induced damage of human umbilical vein endothelial cells
- Author:
Ying CHENG
1
Author Information
1. Department of Endocrinology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(9):957-960
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the protective effect of activated AMP-activated protein kinase (AMPK) on the human umbilical vein endothelial cell (HUVEC) damage induced by palmatic acid (PA) and the related mechanism. Methods: HUVEC were divided into 8 groups according to the culture media (cultured for 24 to 72 h): blank control (conventional culture), 300 μmol/L PA, 1 mmol/L aminoimidazole-4-carboxamide-1-beta-ribofuranoside (AICAR), PA+AICAR, 2 mmol/L metformin, PA+metformin, 10 μmol/L pioglitazone, and PA+pioglitazone groups. Survival rates of HUVEC were determined by MTT at 24, 48 and 72 h after culture. Western blot were performed to detect the expression of phospha-AMPK at 24 h after culture. Results: Compared with blank control group, cell survival rates in PA group were decreased by 58.95%, 36.68% and 15.09% at 24, 48 and 72 h (P<0.05), respectively. Seventy-two hours after culture, HUVEC survival rates of AICAR+PA group, Met+PA group and PGZ+PA group were obviously higher than that of PA group (P<0.05); AICAR, metformin or pioglitazone alone had no obvious influence on HUVEC survival rate. Twenty-four hours after culture, Phospha-AMPK expression was increased in AICAR, metformin and pioglitazone groups compared with that of control group (P<0.05). Conclusion: AICAR, metformin and pioglitazone can decrease the damage of HUVEC induced by PA, possibly through activating AMPK.
