Allogeneic spleen tissue transplantation combined with low-dose FK506 in induction of allograft tolerance in orthotopic liver transplant rats
- Author:
Yong-Zhao SHI
1
Author Information
1. Institute of Organ Transplantation
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(9):946-949
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the influence of allogeneic spleen tissue transplantation with low-dose FK506 on allograft tolerance in rats receiving orthotopic liver transplant and the related mechanism. Methods: Female Brown Norway (BN) rats were divided into 5 groups (eight rats in each group). Rats in Group 1 received orthotopic allogeneic liver transplantation using inbred Lewis rats as donors. Rats in Group 2 received Lewis liver transplantation+low-dose FK-506 (0.25 mg/kg FK-506 was orally given after transplantation). Rats in Group 3 received Lewis liver transplantation+Lewis spleen transplantation. Rats in Group 4 received Lewis liver transplantation+Lewis spleen transplantation+low-dose FK-506. Rats in Group 5 received Lewis liver+SD spleen transplantation+low-dose FK-506. The survival periods were recorded for each group. The forming of chimerism, the responsiveness of T cells to alloantigen and the pathological changes of transplanted liver were analyzed after treatment. Results: Compared with rats in other groups, rats in Group 4 had an obviously longer survival time, an obviously reduced T cell responsiveness to alloantigen, and obviously increased donor-positive cells (remained for 60 days after transplantation) (P<0.05). There was no significant pathological evidence of rejection. Conclusion: Allogeneic spleen transplantation combined with low-dose FK506 treatment can induce graft immune tolerance in orthotopic allogeneic liver transplant recipients through promoting the formation of chimerism and T cells hyporesonsiveness to alloantigen.