Preparation and characterization of amphotericin B-polybutylcyanoacrylate nanoparticles
- Author:
Nan XU
1
Author Information
1. Department of Dermatology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(10):1127-1130
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare amphotericin B (AmB)-polybutylcyanoacrylate (PBCA) nanoparticle and evaluate its characteristics, so as to screen for the optimal preparation technique. Methods: AmB was loaded to blank PBCA nanoparticles by incubation to prepare AmB-PBCA nanoparticles. The surface of the nanoparticles was modified with polysorbate 80. The AmB concentration of AmB-PBCA-NP solution was calculated with the standard curve of AmB at D405, and the stability of solution was assessed. The diameter distribution of the particles was determined with Coulter Laser granulometer. The colloid solution of AmB-PBCA-NP was centrifuged and the supernatant was harvested to determine the concentration of AmB. The encapsulation rate, drug loading rate, and in vitro drug release were measured. The optimized preparation recipe was screened using diameter, drug loading rate, and encapsulation rate of the particles as indices. Results: The prepared AmB-PBCA nanoparticle had a regular spherical or elliptic surface, with a mean diameter of (69.01±28.56) nm. The curve of standard AmB was linear within the range of 1.12-5.60 μg/ml: D405=0.163 4c+0.0066 (r=0.9993). The average recovery of AmB-PBCA-NP was 99.93%, showing the solution of AmB-PBCA-NP was stable within 12 h. The nanoparticles showed a sustained drug release in vitro within 24 h. The optimized recipe was: DextranT-70 stabilizer without sodium deoxycholate, with a mean encapsulation rate of 56.10% and a drug loading rate of 82%. Conclusion: The current method for preparing AmB-PBCA nanoparticles is simple and can meet the requirements of pharmaceutics.
