Gene microarray analysis of peripheral blood mononuclear cells in type 2 diabetes patients with distal symmetric polyneuropathy
- Author:
Xiang LI
1
Author Information
1. Department of Endocrinology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(10):1072-1075
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To screen for the differentially expressed genes in peripheral blood mononuclear cells (PBMCs) from type 2 diabetes patients with distal symmetric polyneuropathy (DSPN) by gene microarray. Methods: A 5075 gene cDNA microarray was used to examine gene expression in PBMCs of 2 type 2 diabetes patients with DSPN (group 1), 2 type 2 diabetes patients without DSPN (group 2),and 2 age- and sex-matched controls (control group). The differentially expressed genes were identified and subjected to real-time RT-PCR analysis. Results: Twenty-two genes were found to be differentially expressed, including cell metabolism and signal transduction genes, oncogenes and anti-oncogenes, DNA synthesis and repair genes, ion channel and transport protein genes, DNA-binding, transcription and transcription factor genes, and cytoskeleton genes. There were 4 genes up-regulated and 18 down-regulated. Real-time RT-PCR results of AK1 and FBX07 were consistent with that of microarray analysis. Conclusion: Microarray expression profile of PBMC in type 2 diabetes patients with DSPN is differential. DSPN may be involved in cell metabolism, signal transduction, DNA synthesis, etc.
