Influence of Retn gene expression on glucose uptake by 3T3-L1 cells and the related mechanism
- Author:
Huai-Xing LI
1
Author Information
1. Institute for Nutritional Science
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(10):1067-1071
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the influence of high expression of Retn gene on glucose uptake by 3T3-L1 cells and to study its mechanism in inducing insulin resistance. Methods: (1) Radioimmunoassay was used to determine the glucose uptake by 3T3-L1 cells with low-, normal- and high-level Retn expression under basal and insulin-stimulated states. (2) RT-PCR and real-time RT-PCR were used to determine the mRNA levels of several glucose transport proteins in 3T3-L1 cells with different expression of Retn, including insulin receptor substrate-1(IRS-1), phosphatidylinositol 3-kinase (PI-3K), AKT-2, glucose transporter-4 (GLUT-4), p38 mitogen-activated protein kinase (p38MAPK), and glycogen synthase kinase-3β(GSK-3β). Results: The uptake of glucose decreased with the increase of Retn expression under basal and insulin-stimulated conditions. The mRNA expression of 2 signal protein PI-3K and AKT-2 decreased with the increase of Retn expression; and the expression of GSK-3β and p38MAPK increased with the increase of Retn expression. Conclusion: Resistin protein can induce insulin resistance in adipocytes, which might be related to the expression changes of some proteins in PI-3K and Ras pathways.
