Influence of a self-designed multiple organ preservation solution on apoptosis of renal cortical cells during hypothermic storage
- Author:
Qiang NIU
1
Author Information
1. Organ Transplantation Institute of PLA
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(10):1059-1062
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the influence of a self-designed multiple organ preservation solution, namely Shanghai multi-organ preservation solution (SMO), on the apoptosis of canine renal cortical cells during cold storage and the related mechanism. Methods: Canine kidneys were freshly perfused and separately stored with cold (0-4°C) SMO, hypertonic adenine citrate solution (HC-A), and University of Wisconsin solution (UW solution) (the latter 2 as control). At defined time points during storage, the morphologic changes of renal cortical samples were observed; apoptosis of renal cortical cell was determined by TUNEL; changes of malonldialdehyde (MDA) in cortex homogenate were measured by thiobarbituric acid method; and the activity of superoxide dismutase (SOD) was determined by xanthine oxidase method. Results: Pathologic assessments indicated that the kidney damage was similar in 3 groups within 24 h. The kidney tubular cell swelling and necrosis in SMO group and UW group were slighter than those in HC-A group after stored for 2 days. With the prolongation of storage time, apoptosis increased in all groups, with the apoptotic index of SMO group significantly lower than that of HC-A group (P<0.05). The levels of cortical MDA increased with storage time, and that in SMO group was significantly lower than that in HC-A group (P<0.05). The activities of cortical SOD were similar in the 3 groups. Conclusion: SMO solution can improve the efficiency for protection of renal cortex, which might be associated with the decrease of cells apoptosis and inhibition of lipid peroxidation injury.
