Preparation and characterization of type III sodium-phosphate cotransporter siRNA-loaded chitosan nanoparticles
- Author:
Dan LI
1
Author Information
1. Department of Nephrology
- Publication Type:Journal Article
- Keywords:
Chitosan;
Nanoparticles;
RNA;
Small interfering;
Sodium-phosphate cotransporter
- From:
Academic Journal of Second Military Medical University
2006;27(11):1258-1260
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare type III sodium-phosphate cotransporter (NaPi-III) siRNA-loaded chitosan microspheres and to evaluate their physico-chemical properties and siRNA release in vitro. Methods: NaPi- III siRNA was loaded into chitosan microspheres by using the complex coacervation method. The structure of the nanoparticles was observed under scanning electron microscope and their diameter distribution was measured by a laser grain analyzer. RNase assay was used to detect the efficacy of anoparticles in prevention of siRNA from degradation. Ultraviolet spectrophotometry and HPLC technique were used to determine the entrapment efficiency, loading capacity, and siRNA releasing rate in vitro. Results: The chitosan nanoparticles loaded with NaPi-III siRNA were successfully prepared. The nanoparticles were spherical in shape and were well distributed, with an average diameter of 173 nm. After RNase treatment, D260 rose more slowly in chitosan nanoparticles-based siRNA suspension than in simple NaPi-III siRNA solution(P<0.05, t = 4.32). Loading capacity of the nanoparticles was 28.1 %, the encapsulation efficiency was 73.07 %, and the total releasing rate of NaPi-III siRNA was less than 20% within 12 hours. Conclusion: Chitosan nanoparticles loaded with NaPi-III siRNA have been prepared through a complex coacervation method. The particles have high encapsulation efficency and good stability and are homogeneous in size. They can obviously delay the release of NaPi-III siRNA in vitro and therefore prevent NaPi-III siRNA from degradation.
