Influence of ischemia/reperfusion on characteristics of membrane potassium channels of human renal tubule epithelial cells
- Author:
Hao CHEN
1
Author Information
1. Department of Urology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(11):1242-1245
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the influence of ischemia/reperfusion (simulated by anoxia) on the characteristics of K+ channels in renal tubular epithelial cells. Methods: Healthy renal tubule epithelial cells were divided into normal control group (cultured in serum-free RPMI 1640 medium), anoxic group (treated with antimycin A + serum-free RPMI 1640 medium), and oxidative stress group (H02 + serum-free RPMI 1640 medium)(n = 15). The whole-cell patch clamp technique was used to examine the characteristics of K+ channels in renal tubular epithelial cells. Results: The transmembrane K+ current in tubular epithelial cells was voltage- dependent and could be blocked by K+ channel blocker Ba2+. The transmembrane potential levels in anoxic group and oxidative stress group were remarkably higher than that in normal control group (P<0.05), while their time constants (r) were smaller than that of normal control group (P<0.05), with no significant difference between the former 2 groups. Conclusion: There are voltage dependent K+ channels on tubular epithelial cells. Their expression increases obviously after ischemia-reperfusion and the activities of the channels also increase obviously.
