Influence of granulocyte-colony stimulating factor combined with adrenocortical steroid and/or immunosuppressants on immune system of donor mice during hematopoietic stem cell mobilization
- Author:
Yong-Bin CAO
1
Author Information
1. Department of Hematology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(11):1200-1203
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the influence of granulocyte-colony stimulating factor (G-CSF) combined with adrenocortical steroid and/or immunosuppressants on immune system of donor mice during hematopoietic stem cell mobilization. Methods: The donor C57BL/6 mice were divided into 4 groups according to the mobilization strategies, namely, the G-CSF group (250 μg/kg for 4 days), G-CSF (250 μg/kg for 4 days) + methylprednisolone group (100 mg/ kg on day 3), G-CSF (250 μg/kg for 4 days) + methylprednisolone group (100 mg/kg on day 3) + cyclosporine A (100 mg/kg on day 3), and PBS control group. The post-mobilization T lymphocytes of C57BL/6 mice and the splenocytes of BALB/c mice were subjected to mixed lymphocyte reactions (MLR). The levels of IFN-γ, IL-4, and TGF-β 1 were determined in the supernatants of donor mice splenocytes by ELISA method and the mRNA levels of IFN-γ and IL-4 were determined by RT-PCR. Results: The results showed that the MLR-inhibitory activities in the 3 G-CSF mobilized groups were 42 %, 21 %, and 39 %, respectively; all lower than that in PBS control group (68%). IFN-γ levels in the 3 G-CSF mobilized groups were lower than that in PBS control group, but he levels of IL-4 and TGF-β 1 were significantly higher than those in PBS group(P<0.01), with no significant difference found between the 3 G-CSF mobilized groups. RT-PCR analysis found that the expression of IFN-y mRNA was obviously decreased and the expression of IL-4 was obviously increased (P<0.01). Conclusion: G-CSF combined with adrenocortical steroid and/or immunosuppressants can decrease the allogenic reaction of T lymphocytes and change the types of secreted cytokines in donor mice during hematopoietic stem cell mobilization, which may induce immune resistance in the recipients.
