Total salvianolic acid inhibiting renal interstitial fibrosis in rats
- Author:
Yan-Jun SHANG
1
Author Information
1. Department of Biochemistry and Molecular Biology
- Publication Type:Journal Article
- Keywords:
Renal interstitial fibrosis;
Total salvianolic acid;
Transforming growth factor-β1
- From:
Academic Journal of Second Military Medical University
2010;27(12):1295-1298
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the influence of total salvianolic acid(TSA) on the renal interstitial fibrosis caused by unilateral ureteral obstruction (UUO) in rats. Methods: Forty healthy Sprague-Dawley rats were randomly divided into 4 groups: the UUO group, sham operation group, angiotensin-converting enzyme inhibitor ( ACEI)-treated group (positive control), and TSA-treated group. The rats in UUO group and sham-operation group received gastric gavage with normal saline for 8 days before operation; rats in ACEI-treated group and TSA-treated group received ACEI (6 mg/kg by daily gastric gavage for 8 days) and TSA (20 mg/kg by daily gastric gavage for 8 days), respectively. All rats were killed 7 days after operation and the expression of transforming growth factor-β 1 (TGF-β1) was detected by immunohistochemical method. The dynamic histological changes of renal interstitial tissues were observed by H-E and Masson staining. Results: TGF-β1 expression in TSA-treated (1.38 ± 0.26) and ACEI-treated (1.38 ± 0.26) groups was significantly lower than that in UUO group (P < 0.05). TSA obviously reduced TGF-β1 expression and collagen deposition in the renal interstitial tissues and improved the renal pathological changes in UUO rats. Conclusion: TSA can evidently inhibit UUO-induced renal interstitial fibros is in rats, which might be related with the down regulation of TGF-β1 expression.
