Preparation and characterization of pulmonary targeting DepoFoam loaded MR contrast media: Gadopentetate dimeglumine
10.3724/SP.J.1008.2008.00087
- Author:
Wei ZHANG
1
Author Information
1. Department of Radiology
- Publication Type:Journal Article
- Keywords:
DepoFoam;
Gd-DTPA;
Lung;
Magnetic resonance imaging
- From:
Academic Journal of Second Military Medical University
2010;28(1):87-90
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare a pulmonary targeting DepoFoam loaded MR contrast media: gadopentetate dimeglumine (Gd-DTPA), and to observe its physi-chemical and biologic characteristics. Methods: The routine magnetic resonance contrast media Gd-DTPA was encapsulated with multivesicular liposome (DepoFoam, Depo) labelled with red fluorescence to obtain a new particle Depo-Gd. The morphology, diameter, and the encapsulation rate of the new particle were examined. The targeting efficiency of the particle was assessed by animal test with mice. Ten mice were evenly divided into 2 groups: one group received caudal vein-injection of Depo-Gd and the other group received vena mesenterica-injection of Depo-Gd. Twenty minutes after the injection the slices of the mouse liver, lung, and kidney were obtained from both group and the fluorescence labeling were observed. Results: Under microscope, the produced Depo-Gd particles were round in shape and stable in status, with good mobility and well-distributed diameter (18 μm in average). The encapsulation rate of the particles was 50% and the concentration of Gd was 0.0417 mmol/ml. The animal fluorescence test showed that mice in the 2 groups were all active and with normal respiratory and conscious status after the injection. In the Depo-Gd caudal vein-injection group, large amount of red dyeing was found in the lung while little red dyeing was found in the liver and kidney; in vena mesenterica-injection group, satisfactory dyeing was found in the liver but not in the lung. Conclusion: The produced Depo-Gd particles have a stable physic-chemical property and with satisfactory encapsulation rate. There is no obvious biological toxicity and allergic reaction. Besides, the particles have, a good pulmonary targeting ability when injected into peripheral veins, making them promising in future clinical application.
