Modification of catalyzed signal amplification method
- Author:
Can-Rong NI
1
Author Information
1. Department of Pathology
- Publication Type:Journal Article
- Keywords:
Catalyzed signal amplification;
Immunohistochemistry;
Sensitivity and specificity
- From:
Academic Journal of Second Military Medical University
2010;28(2):209-211
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To verify whether hot PBS washing after the enzymatic reaction between tyramine and horseradish peroxidase can improve the sensitivity and specificity of catalyzed signal amplification (CSA). Methods: Using 7 different types of primary antibodies and 2 tumor microarray system, we carried out En Vision, LSAB, Standard CSA, and modified CSA staining and compared their sensitivities and specificities. Results: The modified CSA method was the most sensitive one among the 4 methods, followed by standard CSA, En Vision, and LsAB method. The sensitivity of modified CSA method was 2-3 times higher than that of standard CSA. The 4 methods had similar background staining and had exact localization of cells. Conclusion: The modified CSA is more stable and sensitive than traditional CSA staining method.
