Identification and partial purification of antibacterial compounds against Streptococcus mutans from Galla Rhois.
10.11149/jkaoh.2016.40.1.3
- Author:
Ae Ri SHIN
1
;
Seung Ho OHK
;
Choong Ho CHOI
;
Suk Jin HONG
Author Information
1. Department of Preventive & Public Health Dentistry, Chonnam National University School of Dentistry, Gwangju, Korea. sjhong@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
Antibacterial compounds;
Column chromatography;
Galla Rhois;
Partition chromatography;
Streptococcus mutans;
Thin-layer chromatography
- MeSH:
Acetone;
Chromatography;
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Chromatography, Thin Layer;
Dental Caries;
Dimethyl Sulfoxide;
Ethanol;
Methanol;
Solvents;
Streptococcus mutans*;
Streptococcus*;
Water
- From:Journal of Korean Academy of Oral Health
2016;40(1):3-8
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: This study aims to identify and partially purify antibacterial compounds against Streptococcus mutans from Galla Rhois extract. METHODS: Galla Rhois was extracted with n-hexane or ethanol and concentrated in a rotary evaporator. The antibacterial effect of the Galla Rhois extract against S. mutans was determined by the paper discdiffusion method with n-hexane, ethanol, methanol, ethyl acetate, dimethyl sulfoxide (DMSO), acetone, and distilled water as the solvents. The active compounds were purified by partition chromatography, thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC). RESULTS: The antibacterial effect of the n-hexane extract was more effective against S. mutans than the ethanol extract (P<0.05). The antibacterial component of Galla Rhois was partially purified using partition chromatography and HPLC, and the antibacterial activity was confirmed. CONCLUSIONS: The partially purified component of Galla Rhois showed strong antibacterial effect against S. mutans. These results confirm that the antibacterial compounds of Galla Rhois can be used for the prevention of dental caries.
