Polymorphism of adinoponectin gene in pedigree of type 2 diabetes mellitus
- Author:
Ji-Ying WANG
1
Author Information
1. Department of Endocrinology
- Publication Type:Journal Article
- Keywords:
Adiponectin gene;
Diabetes mellitus;
Polymorphism;
Single-nucleotide;
Type 2
- From:
Academic Journal of Second Military Medical University
2010;28(2):143-147
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the correlation between the single nucleotide polymorphism (SNP) of adiponectin (APM1) gene and type 2 diabetes mellitus. Methods: Three common binding sites (-11377C>G, +45T>G, and +276G> T) of SNP on the APM1 gene were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay in 75 pedigrees (337 individuals) of type 2 diabetes mellitus. Genehunt software was used to analyze the transmission-disequilibrium (TDT) and to calculate SNP combining haplotypes. Meanwhile, the physiological and biochemical parameters were also determined in the pedigrees of type 2 diabetes mellitus. Results: We found no preferential transmission in the tested binding sites or any haplotypes of SNP in the APM1 gene in the filial generation of type 2 diabetes mellitus. In type 2 diabetes mellitus group, GG genotype had higher body mass index (BMD (P=O.032) and waist circumference (WC) (P= 0.030) compared to CC genotype in the patients with SNP-11377 binding site; patients with G allele had lower levels of high-density lipoprotein cholesterol (HDL-C) (P = 0.006) and higher levels of fasting plasma insulin (FINS) (P = 0.011) as compared to CC genotype. However, FINS levels (P=O.021) in subjects with CC genotype were significantly lower than those with G allele in healthy control group. For patients with SNP+45 binding site, those with GG genotypes had higher BMI (P= 0.036) and lower levels of FINS (P = 0.014) than those with TT genotypes in both groups. For patients with SNP + 276 binding site, those with GG genotypes had higher BMI(P = 0.043) than those with T allele in the control group. Conclusion: The SNP of the APM1 gene is not associated with the pedigrees of type 2 diabetes mellitus, but APMl gene has influence on the function of insulin B cells and the development of obesity.
