Comparative Study on Expression of Proliferating Cell Nuclear Antigen ( PCNA ) and Ki - 67 Antigen in Several Skin Tumors.
- Author:
Sun Wook HWANG
;
Young Ho WON
;
Inn Ki CHUN
;
Chang Soo PARK
- Publication Type:Comparative Study ; Original Article
- Keywords:
Ki-67;
PCNA;
MIBI;
PC10
- MeSH:
Actins;
Antibodies;
Cell Proliferation;
Humans;
Immunohistochemistry;
Kinetics;
Male;
Proliferating Cell Nuclear Antigen*;
Skin*
- From:Korean Journal of Dermatology
1995;33(3):453-458
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Both PCNA and Ki-67 have been used as marker for cellular proliferation. The drawback of Ki-67 antibody in immunohistochemistry was that it can be labelled only on fresh tissue, however, MIB1 is a newly developed Ki-67 antiboc which can be labelled on formalin-fixed tissue. OBJECTIVE: The purpase of the present study is to compir the stainability of the Ki-67 antibody with that of the ICNA antibody on formalin-fixed, para fin embedded tissues. METHODS: Using MIE1, the newly developed Ki-67 antibody and PC10(PCNA antibody), speci mens of squamous cell carcinoma(SCC), Bowens disease(BL), actinic keratosis(AK) and basal cell epithelioma(BCE) were stained by one hour immunocytial, mistry using a Microprobe immuno/DNA stainer. RESULTS: The labelling indices (LI) of MIB1 were 82.6%, 37.4%, 38.3% & 81.1% respectively in SCC, BD, AK & BC, while the LI of PC10 were 77.69%, 26.6% & 64.4%. The labelled cells of both antibodies differed in distribution patterns on turmor tissues. CONCLUSION: MIBI cain be used to be an alternative m.rl r for proliferating cells. MIBI PC10, when used together, will be mutually compensatory the study of proliferating cell kinetics.
