Detection of in vitro anti-fungal activity of 20 tetralin compounds
- Author:
Rong-Mei LIANG
1
Author Information
1. Department of Pharmacology
- Publication Type:Journal Article
- Keywords:
Anti-fungal agents;
Microbial sensitivity tests;
Tetrahydronaphthalenes
- From:
Academic Journal of Second Military Medical University
2010;28(3):286-293
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the anti-fungal activities of 20 tetralin compounds in vitro. Methods: We adopted the M27-A project recommended by the National Committee for Clinical Laboratory Standard (NCCLS). Tetralin compounds were tested and selected with several candidal strains and non-candidal strains with different susceptibilities to fluconazole. After obtaining the susceptibility results, we plotted the time-growth curves of several typical tetralin compounds, including compound 22-1, 31-1 and their muriate 22, 31, as well as compound 34-1, 26-1 and their bromate 34, 26. The combination effects of compound 22, 26, 34, 31-1 with other anti-fungal agents (with different structures) were also determined. Results: The 20 tetralin compounds were proven to have different degrees of anti-fungal activities. Compound 31-1 had a stronger anti-fungal activity to FLC-susceptible strains than fluconazole did, and its effect lasted for over 54 h at the concentration of 6 μg/ml. Compounds 22-1 and 22 markedly suppressed the growth of Candida albicans, Candida parapsilosis, and Trichophyton rubrum, with all the MIC50 values less than 0.125 μg/ml. Time-growth curves indicated that the anti-fungal activity of 22-1 on fluconazole-resistant strain was more powerful than that of ketoconazole. Furthermore, tetralin compounds had a synergistic effect with terbinafine and berberine on fluconazole-resistant strains. Conclusion: Tetralin compounds have powerful anti-fungal activities and their structures are different from those of other anti-fungal agents currently used, which provide a basis for developing new anti-fungal agents.
