Preparation of amphotericin B-polybutylcyanoacrylate-loaded nanopaticles and its distribution in mice
- Author:
Nan XU
1
Author Information
1. Department of Dermatology
- Publication Type:Journal Article
- Keywords:
Amphotericin B1;
Blood-brain barrier;
Meningitis, cryptococcal;
Polybutylcyanoacrylate
- From:
Academic Journal of Second Military Medical University
2010;28(3):242-245
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare amphotericin B-polybutylcyanoacrylate-loaded nanoparticle (AmB-PBCA-NP) and to assess their ability to pass the blood-brain barrier. Methods: AmB-PBCA-NP was prepared by incubation, with the surface modified by polysorbate 80. High-performance liquid chromatography (HPLC) method was established using acetonitrile and water (40:60, 4% of acetic acid) as mobile phase; detection was done at 405 nm. Mice were divided into 3 groups: AmB-injected, AmB-lipid-injected, and AmB-PBCA-NP-injected group. Brain-targeting ability of AmB-PBCA-NP was then evaluated by determination of AmB concentrations in mice brain and other specimens. Results: The mean diameter of the prepared AmB-PBCA-NP was 94.38 nm; the mean entrapment efficiency of the particles was 82%; and the drug loading rate was 56.10%. It was found that AmB alone failed to pass the blood-brain barrier; only very low level of AmB was detected in AmB-lipid group 3 h after injection; moderate concentration of Amb was detected 30 min after injection of AmB-PBCA-NP and the concentration peaked at 133 ng/g 3 h after injection. Conclusion: AmB-PBCA-NP modified by polysorbate 80 can pass the blood-brain barrier.
