Transplantation of autologous sinoatrial nodal cells in treatment of heart block
- Author:
Hao ZHANG
1
Author Information
1. Department of Cardiothoracic Surgery
- Publication Type:Journal Article
- Keywords:
Cell transplantation;
Heart block;
Sinoatrial node;
Transplantation, autologous
- From:
Academic Journal of Second Military Medical University
2010;28(3):233-236
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop a novel method for treating complete heart block by transplanting autologous sinoatrial node(SAN) cells to the right ventricle adjacent to heart apex. Methods: Twelve young pigs were evenly randomized into transplantation group and control group. The sinoatrial nodes of all pigs were harvested and SAN cells were isolated in vitro. Pigs in transplantation group were injected with a cell suspension and those in control group were injected with culture medium into autogeneic right ventricular wall adjacent to heart apex after the temporary myocardial pacing wires were fixed. Two weeks later, all pigs underwent electrophysiology mapping and transcatheter ablation of His bundle to create a complete heart block model. The heart rates of pigs in both groups were observed after isoproterenol (ISO) being injected through femoral vein. Results: The isolated cells had good viability and all the pigs showed complete heart block ECG waveforms after ablation. Ventricular autonomic rhythms of pigs in transplantation group were faster than those of pigs in control group (P<0.05). We proved that the rhythms originated from the injected site by endocardial pace mapping. The ventricular rhythms of transplantation group changed remarkably after ISO was administrated (P<0.05). Conclusion: Autogeneic transplantation of SAN cells isolated from young pigs into right ventricular wall adjacent to heart apex can increase the ventricular autonomic rhythm of complete heart block, and this increased rhythm is sensitive to isoproterenol.
