Establishment and optimization of two-dimensional gel electrophoresis condition for Candida albicans proteome analysis
- Author:
Lan YAN
1
Author Information
1. Department of Pharmacology
- Publication Type:Journal Article
- Keywords:
Candida albicans;
Electrophoresis;
Gel;
Proteome;
Two-dimensional
- From:
Academic Journal of Second Military Medical University
2010;28(4):438-441
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop and optimize a two-dimensional(2-DE) gel electrophoresis condition for Candida albicans proteome analysis. Methods: Total cellular protein of Candida albicans strain SC5314 was extracted and subjected to 2-DE under different conditions. The optimal conditions for sample preparation and 2-DE were screened by comparing the following parameters: cell broken methods, contents of lysis buffer, on-gel protein contents, and linear or non-linear immobilized pH gradient gels. Results: We successfully established the method for Candida albicuans protein sample preparation and optimized 2-DE condition for Candida albicans proteome analysis. A satisfactory 2-DE map with good repetition and resolution was obtained. Conclusion: The optimized condition of 2-DE in this article is suitable for Candida albicans strain, which paves a way for analysis of global protein profile in Candida albicans.
