Influence of stress on maintaining pH gradients of rat gastric mucus
- Author:
Xian-Bao ZHAN
1
Author Information
1. Department of Gastroenterology
- Publication Type:Journal Article
- Keywords:
Gastric acid;
Gastric mucosa;
Mucus;
Stress
- From:
Academic Journal of Second Military Medical University
2010;28(4):377-380
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the influence of stress on maintaining pH gradients of rat gastric mucus. Methods: Antimony microelectrodes were prepared according to the literature reported. The pH-mV curves of antimony microelectrode in different buffers were plotted. Forty rats were evenly randomized into 4 groups, including control group (without stress), 1 h-, 2 h- and 4 h- stress groups. Rats in the latter 3 groups received water immersion restraint stress. The gastric cavities in 4 groups were lavaged with normal saline, followed by lavage with 2 ml hydrochloric acid solution (0.01 mol/L) for 10 min, then the glandular mucosa of stomach was sampled and their mucus pH gradients were measured by antimony microelectrodes. Results: The voltages determined by antimony microelectrode in different buffers with the same pH value were different, but they showed a linear correlation with pH-mV in the same buffer. Compared with control group, the 1 h-stress, group had an increased pH gradient (P<0.05) and a decreased luminal-mucus interface pH value (P<0.05); the above 2 groups had a similar mucus-epithelial interface pH value (P>0.05). Luminal-mucus interface pH value in 2 h-stress group was similar to that in the control group (P>0.05), but was higher than that of 1 h-stress group (P<0.05). Compared with 2 h-stress group, the mucus-epithelial interface pH value and the pH gradient in the control and 1 h-stress group were significantly higher (P<0.01). The luminal-mucus interface pH value in the 4 h-stress group was significantly higher than those of the other 3 groups (P<0.05). The mucus-epithelial interface pH in the 4 h-stress group was similar to that in the 2h-stress group, but was lower than those of the control and 1 h-stress groups (P<0.01). The pH gradient in the 4 h-stress group was markedly lower than those in the other 3 groups (P<0.01). Conclusion: The results suggest that the gastric mucosa has an obviously improved ability in maintaining mucus pH gradient during the early stage of stress, and a decreased ability during the later stage of stress. The decrease of mucus-epithelial interface pH value during the late stage of stress indicates that the gastric mucosa undergoes obvious acidosis.
