Nitric oxide content and blood flow in nasal mucosa of allergic rhinitis guinea pigs
- Author:
Su-Min WANG
1
Author Information
1. Department of Otolaryngology
- Publication Type:Journal Article
- Keywords:
Blood flow;
Nasal mucosa;
Nitric oxide;
Rhinitis, allegic, perennial
- From:
Academic Journal of Second Military Medical University
2010;28(5):512-515
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To examine the expression of nitric oxide synthase (NOS), the content of nitric oxide and blood flow in nasal mucosa of allergic rhinitis guinea pigs, so as to further investigate the mechanism of allergic rhinitis. Methods: One hundred and twenty guinea pigs were randomly divided into control group (injected with normal saline) and allergic group (nasal challenge with egg albumin). The guinea pigs were executed before and immediately, 24, 48, 72 h after the last nasal challenge; the expression of endothelial nitric oxide synthase (eNOS)and inducible nitric oxide synthase (iNOS) and the content of nitric oxide were examined in mucosa tissues. The blood flow in the nasal mucosa was determined in animals before execution. Linear regression correlation was used to analyze the relationship between the nitric oxide content and blood flow in nasal mucosa. Results: The immunostaining for iNOS in surface epithelium of allergic rhinitis guinea pigs was markedly stronger than that of normal guinea pigs at all time points (P<0.01); immunostaining of eNOS was similar in the 2 groups. Compared with those in normal guinea pigs, the content of NO and blood flow increased significantly before the last challenge with allergen (P<0.01), decreased significantly immediately after the last nasal challenge (P<0.01), and then increased gradually and restored the normal level 72 h later; there was no significant difference in control group before and after challenge. The content of NO and blood flow in nasal mucosa was linearly correlated with each other in allergic group (P<0.01). Conclusion: Overexpression of iNOS protein in the nasal mucosa of allergic guinea pigs may increase local NO content and subsequently increase the blood flow in local nasal mucosa.
