Expression of Bcl-2 and Bax in renal tissues of patients with hepatitis B virus-associated glomerulonephritis
- Author:
Li-Jing SUN
1
Author Information
1. Department of Nephrology
- Publication Type:Journal Article
- Keywords:
Bax;
Bcl-2;
Hepatitis B virus;
Nephritis
- From:
Academic Journal of Second Military Medical University
2010;28(5):492-494
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression of Bcl-2 and Bax in renal tissues of patients with hepatitis B virus-associated glomerulonephritis (HBV-GN). Methods: Twenty HBV-GN specimens with complete nephrology data and 10 normal renal specimens were randomly chosen for the present study. Cell apoptosis was detected by means of terminal deoxynucleotidyl transferase mediated d-UTP nick end labeling (TUNEL) and the apoptotic index was calculated; immunohistochemistry was used to detect the protein expression of Bax and Bcl-2. Results: The apoptotic index in HBV-GN group was obviously higher than that of the control group; the apoptotic cells were mainly distributed in the proximal and distal renal tubules and the collecting duct epithelial cells, seldom seen in the glomerular cells. The expression of Bcl-2 in HBV-GN patients was predominately present in the renal tubular epithelia cells (positive in the plasma, membrane and nuclear); the expression of Bax was found in both glomerular cells and renal tubular cells, mainly in tubular epithelial cells, seldom seen in Bowman's capsule or glomerular mesangial region. Conclusion: Apoptosis in the kidney of HBV-GN patients mainly occurs in the renal tubular epithelial cells; expression of Bax and Bcl-2 is mainly in the renal tubular epithelial cells, suggesting that the injury of tubular interstitial damage may be one of the important factors for the development of HBV-GN.
