Effect of nerve growth factor on denervated bone fracture healing in rats
- Author:
Cheng MA
1
Author Information
1. Department of Orthopaedics
- Publication Type:Journal Article
- Keywords:
Fracture healing;
Nerve growth factor;
Spinal cord injuries
- From:
Academic Journal of Second Military Medical University
2010;28(5):474-479
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of nerve growth factor (NGF) on bone fracture healing of inflicted T10 spinal cord injury (SCI) complicated with tibia fracture in rats. Methods: Totally 120 rats were randomly divided into tibia fracture group (F group, n=40), T10 SCI + tibia fracture group (FS group, n=40), and T10 SCI + tibia fracture + NGF group (FSN group, n=40). Four weeks later, the fracture sites in the 3 groups were subjected to CT scanning; the maximum transverse diameter of the fracture ends and the gray scales of non-osseous area were measured; the changes of biomechanics property of the fracture ends were determined by three-point bending test; the bone morphometry, bone density, and histomorphology of callus were determined; the expression of OCN was detected by immunohistochemical method; the osteoblast ultrastructure was observed by TEM and the expression of I, II type collagen were examined by Western blotting. Results: The maximum transverse diameter of F group was less than those of FS group(P<0.01)and FSN group (P<0.05), and FS group was longer than FSN group(P<0.05); the comparison of gray scales among 3 groups had a similar result to that of the section diameter. Three points bending test and bone morphometry test showed that the biomechanics property of the fracture ends in F group were better than those of the other 2 groups (P<0.05), and FSN group was better than FS group (P<0.05). The expression of OCN and type I collagen in the callus of F group was significantly higher than that of the other 2 groups (P<0.05), and FSN group was higher than FS group (P<0.05); while the expression of type II collagen in F group was lower than that of the other 2 groups(P<0.05), and FSN group was higher than FS gourp (P<0.05). Morphological and ultrastructure observation showed that fracture in F group had the best healing result, and the healing result of FSN group was better than that of the FS group. Conclusion: It is suggested that denervation can affect the ossification of fracture ends and NGF can promote the ossification of fracture ends suffering denervation.
