Effects of propofol on isolated heart function and metabolism of oxygen free radical in rats wth obstructive jaundice
- Author:
Hong-Mei REN
1
Author Information
1. Department of Anesthesiology
- Publication Type:Journal Article
- Keywords:
Heart function;
Malondialdehyde;
Obstructive jaundice;
Propofol;
Superoxide dismutase
- From:
Academic Journal of Second Military Medical University
2010;28(6):640-643
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of propofol of clinical concentration on isolated heart function and metabolism of oxygen free radicals in rats with obstructive jaundice. Methods: Thirty-six rats were randomly divided into 2 groups (n = 18): sham operated group (SO) and bile duct ligation group (BDL). The isolated working hearts were perfused for 30 min and 6 hearts were randomly selected from each group to measure the contents of basic MDA and SOD activity in the heart tissue. The rest animals of each group were randomly separated into 2 subgroups (n = 6): K-H solution perfusion group and propofol perfusion group. The isolated working hearts in K-H solution perfusion group were perfused with K-H solution for 40 min. The hearts in propofol perfusion group (SOP, BDLP) were perfused for 40 min with K-H solution containing 25 μmol/L propofol. After the hearts became stable, the following indices were recorded: HR, left ventricular developed pressure (LVDP), ± dp/dtmax, etc. The contents of MDA and SOD activity were measured after perfusion. Results: After perfusion with K-H solution for 30 min, the basic indices of heart function of BDL group were significantly lower than those of SO group (P<0.05). The indices significantly decreased after perfusion with propofol (P<0.05), but the rates of change of all indices were not different between SO and BDL groups. The contents of basic MDA in BDL groups were significantly higher than those of SO groups (P<0.05); the basic SOD activities in BDL were significantly lower than those of SO groups (P<0.05). After propofol perfusion, MDA content of BDLP group significantly decreased (P<0.05) and SOD activity significantly increased (P<0.05). Conclusion: Propofol at clinical concentration shows no significantly different effects on isolated working heart function of rats with and without obstructive jaundice, which might be related to the fact that propofol can protect SOD activity, lower MDA content and improve the metabolism of myocardial.
