Influence of glycogen synthase kinase 3β overexpression on function of murine macrophage RAW264.7
- Author:
Yuan-Yuan LI
1
Author Information
1. Department of Biochemical Pharmacy
- Publication Type:Journal Article
- Keywords:
Glycogen synthase kinase 3β;
Macrophages;
Nitric oxide;
Phagocytosis;
Tumor necrosis factor
- From:
Academic Journal of Second Military Medical University
2010;28(7):697-700
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the influence of glycogen synthase kinase 3β (GSK3β) overexpression on the function of murine macrophage RAW264.7. Methods: Eucaryotic vector pcDNA3. 1-GSK3β was constructed and transfected into RAW264.7 cells via lipofectamine 2000. RAW264.7 cells stably expressing GSK3β were screened by G418 selection. Phagocytosis ability of RAW264.7 cells was assessed by neutral red analysis; the content of nitric oxide (NO) and the activity of tumor necrosis factor (TNF) in the supernatant of RAW264.7 cells stably expressing GSK3β were determined by Griess method and crystal violet staining assay, respectively. Results: RAW264.7 cells stably expressing GSK3β showed an inhibited phagocytosis ability, with the inhibitory rate being 20.4% (P<0.05), and an inhibited production of NO by RAW264.7 cells stably expressing GSK3β in presence of 1 μg/ml LPS, with the inhibitory rate being 25.6% (P<0.01). The transfected RAW264.7 cells had an increased production of TNF by 28.8% (P<0.01). Conclusion: GSK3β can regulate macrophages in inflammatory and immune responses.
