Isolation, culture and identification of adipose derived stem cells from human subcutaneous adipose tissues
- Author:
Xun-Yi ZHANG
1
Author Information
1. Department of Histology and Embryology
- Publication Type:Journal Article
- Keywords:
Adipose tissue;
Cell separation;
Mesenchymal stem cells;
Tissue engineering
- From:
Academic Journal of Second Military Medical University
2010;28(8):813-816
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for isolating adipose derived stem cells (ADSCs) from resected human subcutaneous adipose tissues. Methods: ADSCs were isolated, cultured, and expanded from human subcutaneous adipose tissues. Immuno-fluorescent staining of specific molecules, FACS and multi-lineage differentiation induction were used to characterize the obtained ADSCs. Results: ADSCs obtained in this study had the characteristics of stem cells and expressed specific molecules; they also possessed a multi-lineage differentiation potential,which was genetically stable. Conclusion: ADSCs can be isolated from human subcutaneous adipose tissues, which provides a novel and abundant seeding cells for tissue engineering.
