Expression of MMP-2, MMP-2, VEGF and RECK genes in bladder cancer and its clinical significance
- Author:
Yang YAN
1
Author Information
1. Department of Urology
- Publication Type:Journal Article
- Keywords:
Bladder neoplasms;
Expressions of multiple gene;
Neoplasms metastasis
- From:
Academic Journal of Second Military Medical University
2010;28(10):1075-1078
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the expression of MMP-2, MMP-9, vascular endothelial growth factor (VEGF) and RECK genes in bladder cancer tissues, so as to provide evidence for studying the molecular mechanism of bladder cancer metastasis. Methods: Reverse transcription polymerase chain reaction (RT-PCR) was used to determine the expression of MMP-2, MMP-9, VEGF and RECK genes in 50 operative specimens of bladder cancer and their corresponding adjacent normal tissues; and the clinical significance of the expression was analyzed. Results: The positive rates of MMP-2, MMP-9, VEGF and RECK mRNA in the bladder cancer specimens were 80%, 56%, 78% and 64%, respectively. The expression levels of MMP-2, MMP-9 and VEGF mRNA in bladder cancer specimens were significantly higher than those in the paired adjacent normal bladder tissues(P<0.05); the expression level of RECK genes in bladder cancer specimens was significantly lower than that in the normal tissue (P<0.05). The expression levels of MMP-2, MMP-9 and VEGF was closely correlated with the clinical stages and grades of bladder cancer; there was no relation between the level of RECK expression and clinical stages and grades of the cancer. Conclusion: Abnormal expression of multi-genes is present in bladder cancer tissues; the development, progression and metastasis of bladder cancer are associated with abnormal expression of multi-genes.
