Abnormal methylation of RASSF1A and BLU genes in renal carcinoma
- Author:
Jian-Min DUAN
1
Author Information
1. Department of Urology
- Publication Type:Journal Article
- Keywords:
BLU;
DNA methylation;
Genes;
Kidney neoplasms;
RASSF1A;
Tumor suppressor
- From:
Academic Journal of Second Military Medical University
2010;28(10):1068-1071
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the methylation status of the promoters of RASSF1A and BLU genes in the renal carcinoma tissues and to assess their roles in the tumorigenesis of renal carcinoma. Methods: Methylation specific PCR (MSP) method was used to examine the methylation status of the promoters of RASSF1A and BLU genes in the renal carcinoma tissues of 26 patients with renal carcinoma and their corresponding adjacent normal tissues; and the results were analyzed. Results: We found that 17 (65.4%) of the renal carcinoma tissues had abnormal methylation of RASSF1A gene, and there was no abnormal methylation of RASSF1A in the adjacent normal tissues. Eleven(42.3%) of 26 renal cancer tissues had hypermethylation of BLU gene, and there was no hypermethylation of BLU gene in the corresponding adjacent tissues. Conclusion: Hypermethylation of RASSF1A and BLU genes is present in the renal carcinoma tissues, suggesting the hypermethylation of RASSF1A and BLU might be associated with the tumorigenesis of renal carcinoma.
