Melatonin inhibits the regulation of hypothalamo-pituitary-adrenal axis in diabetic rats
- Author:
Ling XIA
1
Author Information
1. Department of Endocrinology
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus, experimental;
Hypothalamo-pituitary-adrenal axis;
Melatonin
- From:
Academic Journal of Second Military Medical University
2010;28(11):1184-1187
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of melatonin (Mel) , a potent antioxidant, on the oxidative stress and the activity of hypothalamo-pituitary-adrenal axis in diabetic rats. Methods: Male SD rats were assigned to the following 4 groups: normal control (treated with 2% ethyl alcohol lavage[ NC, n=6 ]), diabetes mellitus control (treated with 2% ethyl alcohol lavage [DM, n=7]), diabetes mellitus model group 1 (treated with Mel lavage [10 mg · kg-1 d-1, DM+Mell, n=61), diabetes mellitus model group 2 (treated with Mel lavage [0. 2 mg · kg-1 · d-1 , DM+Me2, n=61). Diabetes in DM group, DM+Mell group and DM+Mel2 group was induced by intraperitoneal injection of streptozotacin (65mg/kg). Blood glucose, body weight and adrenal gland weight/body weight(AW/BW) were determined in the 4 groups 12 weeks later; malondialdehyde (MDA) was examined with visible spectrophotometry; blood total cholesterol (TC) and triglyceride (TG) were examined with enzymology method; blood corticotrophin-releasing hormone (CRH), adreno-corticotrophic hormone(ACTH) and corticosterone(CORT) were examined with radioimmune method. Results: Blood glucose, MDA , TC, TG, CRH, ACTH, CORT and AW/BW in DM group were significantly higher than those in NC group (P<0.01); and the body weight of rats in DM group were significantly lower those of NC group(P<0.01). Compared with DM group, DM+Mel1 and DM+Mel2 group had a significantly lower blood glucose, MDA TC, TG and AW/BW (P<0.01 or P
