Expression of N-glycome in sera of patients with hepatocellular carcinoma
- Author:
Yun-Peng ZHAO
1
Author Information
1. Department of Laboratory Medicine
- Publication Type:Journal Article
- Keywords:
Carcinoma, hepatocellular;
Electrophoresis, capillary;
N-glycome
- From:
Academic Journal of Second Military Medical University
2010;28(12):1280-1283
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To compare the expression profiles of serum glycomics between hepatocellular carcinoma(HCC) patients and normal adults, in an attempt to search for new biomarkers for HCC. Methods: Serum samples were obtained from HCC patients with hepatitis B virus(HBV) infection (n = 100) and normal adults(n = 130). Profiles of serum N-glycome were determined by capillary electrophoresis (CE) based on DNA sequencing equipment. Results: Each sample had a profile with 11 peaks; the peaks were adjusted and quantified. Peaks 1, 2, 9, and 11 in HCC group were higher than those in the control group; peaks 6, 8 and 10 were lower in HCC group than those in control group (P<0.05). The changes of peak values of several peaks were correlated with tumor markers such as alpha-fetoprotein(AFP), HBV DNA copies, albumin and prothrombin time. Conclusion: N-glycome profiles in serum are significantly different between HCC patients and normal adults, which may provide evidence for clinical diagnosis of HCC.
