A method for detection of HER2 gene amplification in breast cancer tissues: Chromogenic in situ hybridization method and its modification
10.3724/SP.J.1008.2008.00099
- Author:
Can-Rong NI
1
Author Information
1. Department of Pathology
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
c-erbB-2 gene;
Chromogenic in situ hybridization;
Human epidermal growth factor receptor 2
- From:
Academic Journal of Second Military Medical University
2010;29(1):99-102
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To apply chromogenic in situ hybridization (CISH for detection of HER2 gene amplification in breast cancer tissues and to discuss some modifications of the CISH method. Methods: HER2 gene amplification was detected by CISH in 60 breast cancer specimens with an immunohistochemical score over 2+. The correlation between the results of IHC and CISH was analyzed. Our experience in CISH manipulation was summarized and optimization to CISH was discussed. Results: CISH identified gene amplification in 91% (40/44) specimens with an IHC score of 3+ and in 50% (8/16) specimens with an IHC score of 2+. The total concordance rate between IHC and CISH was 80% (48/60, P<0.01). The thickness of sections should be controlled within 4-5 μm; the denaturation should be complete; and the post-hybridization washing temperature and time were also very important and the temperature should be controlled at 70-75 °C. The dyeing time of hematoxylin should also be restrictedly controlled. Positive control should be set up in the experiment for high quality of the experiment. Conclusion: CISH has high concordance rate with IHC in examining HER2 amplification and it may be a new method for detection of HER2 gene. The thickness of the sections, the post hybridization washing temperature and time, and the time of hematoxylin dyeing should be strictly controlled.
