Analysis of polymorphism in human cDNA sequence of prothymosin-α
10.3724/SP.J.1008.2008.00092
- Author:
Xue-Lian GONG
1
Author Information
1. Department of Biochemical Pharmacy
- Publication Type:Journal Article
- Keywords:
cDNA;
Prothymosin-α;
Sequence analysis
- From:
Academic Journal of Second Military Medical University
2010;29(1):92-94
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyze the polymorphism in human cDNA sequence of prothymosin α (ProTα) by sequencing analysis. Methods: The cDNA of human ProTα was amplified from cells of peripheral blood and cord blood by RT-PCR. The product of RT-PCR was purified and linked with vector pMD18-T. After cloning and sequencing, the sequence of ProTα cDNA was compared with the standard sequence to analyze the polymorphism in the ProTα cDNA sequence. Results: The cloned ProTα cDNA sequence was different from that of the standard. We found 2 kinds of variations: (1) The nucleotide in 107 position was varied and the nucleotides in 110-121 and 191-205 positions were deleted; (2) The nucleotide in 306 position was deleted, mainly in the 60-80 years old group. Conclusion: We have identified 2 kinds of variations in human ProTα cDNA, but the first 28 amino acid in the N-terminal of cDNA of human ProTα are not involved therefore the variations do not affect the function of human ProTα.