Comparison of haemolytic activities of venom separations from jellyfish Cyanea capillata and their influencing factors
10.3724/SP.J.1008.2008.00083
- Author:
Fei NIE
1
Author Information
1. Department of Chemical Defense Medicine
- Publication Type:Journal Article
- Keywords:
Cyanea capillata;
Haemolysis activity;
Nematocyst venom;
Tentacle-only extract of jellyfish
- From:
Academic Journal of Second Military Medical University
2010;29(1):83-86
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To separate the nematocyst venom (NV) and tentacle-only extract (TOE) from the jellyfish Cyanea capillata and to analyze the difference between their haemolytic activities and the influencing factors. Methods: NV and TOE were separated by autolysis and centrifugation. The influence of concentration, temperature and pH value on the haemolytic activities of NV and TOE were observed. Results: NV and TOE were successfully separated. The concentration-associated haemolytic curves were "S" shaped for both NV and TOE. The HU50 of NV and TOE were 8 μg/ml and 67 μg/ml, respectively; and the haemolysis of NV was about 8.4 times of that of TOE. Temperature had great influence on haemolysis of both and the highest haemolysis were both at 40°C. pH value also had influence on haemolysis. The strongest haemolysis activity was found both at pH 8 and TOE was more sensitive. Conclusion: NV and TOE both have haemolysis activity, with the activity of NV stronger than that of the latter. The haemolysis activity is influenced by concentration, temperature and pH value.
